Purification of Synechococcus lividus by Equilibrium Centrifugation and Its Synchronization by Differential Centrifugation

Purification of Tomato Bushy Stunt Virus by Differential Centrifugation

Purification of bacterial endotoxins by zonal centrifugation.

In this paper, we describe a cultivation procedure in large-capacity fermentors (200 liters) and the purification of the Salmonella crude extracts by means of zonal centrifugation in a sucrose gradient. Briefly, the endotoxin has been extracted from salmonellae with hypertonic solutions. The crude endotoxin was shown to contain several antigens, mainly r(1), r(2), r(3), or heterologous antigen....

Immunoassay of digoxin by differential centrifugation.

We describe an immunoassay of digoxin that exploits the differential sedimentation rate between two types of latex particles in a microcentrifugal analyzer. Conventional polystyrene latex particles (relative density = 1.05), sensitized with antibody, are first suspended with the sample and then mixed with digoxin-sensitized latex particles (relative density = 1.5). In the absence of digoxin in ...

Concentration and purification of poliovirus by ultrafiltration and isopycnic centrifugation.

A method is described by which poliovirus can be rapidly and simply concentrated by the use of a Diaflo XM-50 ultrafilter membrane. Freon-extracted ultrafilter concentrates banded in CsCl provided a 1,724-fold volumetric concentration of poliovirus. During concentration, trypsin-digested cellular material can pass through the ultrafilter membrane, thus providing a versatile means of degrading a...

Purification of Closed Circular DNA by Equilibrium Centrifugation in CsCl-Ethidium Bromide Gradients: Discontinuous Gradients.

A. Continuous Gradients 1. Measure the volume of the DNA solution. For every milliliter, add exactly 1 g of solid CsCl. 2. Warm solution to 30C to facilitate the dissolution of the salt. Mix until dissolved 3. Add 0.8 mL of ethidium bromide (10 mg/mL in water) for every 10 mL of DNA/CsCl solution. 4. Mix the ethidium bromide solution with the denser DNA/CsCl solution. 5. The final density shoul...